The effective way of control cancers currently relies on early case finding and treatment but the best way is more fundamental prevention through inhibition of many stages of carcinogenesis.
Since many cancers are related to environmental and life style factors, recently chemoprevention through micronutrients, nonnutrient plant substances, synthetic nutrient derivatives, and synthetic chemicals have been attracted interest of cancer
researcher. Almost 40clinical chemoprevention trials are now in progress around world among which γcarotene and 13cis retinoic acid were proven to be effetive in many cancers including colon, lung, breast, skin, cervix and head and neck cancer.
The main purpose of this study is to get a basic data for the development of chemopreventive agents in Korean Herbs.
It has been reported that Ganoderma has a hypotensive effect through the inhibition of angiotensin converting enzyme, antiallergic and antiasthmatic effect through the inhibition of histamin release from the mast cells, in addition to an
inhibitory
effect of lipid peroxidation, and a potenting effect of the immune system.
Attempts were made to see the anticancer effect of Ganoderma extracted with hot water a common preparative method in most herb medicine, employing human cancer cell lines such as K562 derived from erythroleukemia, Raji from lymphoma and Molt4
from
blastogenic tumors.
Apart from those tumor cell suspinsions derived from hematopoietic cells, the anticancer effect of Ganoderma was also investigated with other human solid tumors of liver, lung, kidney and stomach cancers.
The anticancer effect of Ganodrma of three different preparations, that is, a naturally grown one, a cultivated one and a molded one during malstorage, was also compared.
@ES The results obtained from this study were as follows.
@EN 1. Inhibitory effect of cancer cell growth by Ganoderma in 5different human solid tumor cell lines such as KHOS/MP, SDK, HeLa, A549, HepG2 was 6-28% showing highest effect in HeLa and lowest effect in HepG2 among tested.
2. Native Ganoderma showed 7-28%, inhibitory effect of cancer cell growth in 5different human solid tumor cell lines tested, and cultivated Ganoderma showed 6-28%, indicating that native and cultivated one do not reveal the difference on the
cancer
cell inhibitory effect. Mal-stored Ganoderma with fungal contanimation showed only 0-12%, half value of cancer cell inhibitory effect.
3. On the contrary to the solid human cancer cell lines, cancer cell suspensions like K562 derived from chronic myelogenous leukemia showed 72% Molt4 derived from acute lymphoblastic leukemia showed 71%, and Raji derived from Burkitt lymphoma
showed
58%, indicating that Ganoderma has more anticancer effect on cancer cell suspensions derived from hematopoietic system than solid tumors tested in this study.
4. In the dosage dependent study of Ganoderma together with 5-fluorouracil(5 FU) as referenfe drug, HeLa cell was most effective showing 30% of cancer cell cytotoxicity at the 1mg/ml, while 5FU showed 47% of cancer cell cytotoxicity at the same
concentration.
Above results revealed Ganoderma was more effective to the cancer cell suspensions derived from hematopoietic system than solid tumors. And cancer cell cytotoxicity is not almost different both in native and cultivated Ganoderma.
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